Collaborative subcellular compartmentalization to improve GPP utilization and boost sabinene accumulation in Saccharomyces cerevisiae

Screening and directed evolution of transporters to improve xylodextrin utilization in the yeast Saccharomyces cerevisiae

Screening of transporters to improve xylodextrin utilization in the yeast Saccharomyces cerevisiae

The economic production of cellulosic biofuel requires efficient and full utilization of all abundant carbohydrates naturally released from plant biomass by enzyme cocktails. Recently, we reconstituted the Neurospora crassa xylodextrin transport and consumption system in Saccharomyces cerevisiae, enabling growth of yeast on xylodextrins aerobically. However, the consumption rate of xylodextrin ...

Growth and feed utilization of goldfish (Carassius auratus) fed graded levels of brewers yeast (Saccharomyces cerevisiae)

In this study, a feeding trial was conducted to examine the potential of replacing fish meal with brewers yeast in practical diet of goldfish (Carassius auratus). Five isoproteic (37% CP) and isocaloric (3350 kcal/kg) diets were formulated to contain graded levels of brewers yeast. Fish meal protein was replaced by 0%, 15%, 25%, 35%, and 45% of yeast. Each diet was randomly allocated to triplic...

Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae

Xylose utilization is one key issue for the bioconversion of lignocelluloses. It is a promising approach to engineering heterologous pathway for xylose utilization in Saccharomyces cerevisiae. Here, we constructed a xylose-fermenting yeast SyBE001 through combinatorial fine-tuning the expression of XylA and endogenous XKS1. Additional overexpression of genes RKI1, RPE1, TKL1, and TAL1 in the no...

Growth and feed utilization of goldfish (Carassius auratus) fed graded levels of brewers yeast (Saccharomyces cerevisiae)

In this study, a feeding trial was conducted to examine the potential of replacing fish meal with brewers yeast in practical diet of goldfish (Carassius auratus). Five isoproteic (37% CP) and isocaloric (3350 kcal/kg) diets were formulated to contain graded levels of brewers yeast. Fish meal protein was replaced by 0%, 15%, 25%, 35%, and 45% of yeast. Each diet was randomly allocated to triplic...